Crude oil and pyrene degradation by halotolerant fungi <i>Embellisia</i> sp. KJ59 and <i>Alternaria</i> sp. KJ66 isolated from saline soils

In recent years, investigating halophilic and halotolerant fungi enzymes has confirmed their significant potential in the biodegradation of crude oil. The aim current study was to evaluate oil by fungal Embellisia sp. KJ59 Alternaria KJ66. strains were isolated on Czapek Dox Agar (CDA), potato dextrose agar (PDA), malt extract (MEA) containing 10% NaCl. Seven that could grow salinity, ranging from 0 17% selected. Out seven isolates, isolates KJ66 selected as superior because they more efficient Minimal Salt Medium 1% different salinity. identified based its nucleotide sequence internal transcribed spacer gene. They biodegrade petroleum, 81.3 95.1% NaCl concentrations 0, 2.5, 5% PDB over a period 14 days. Pyrene removal these 88.3% saline non-saline MSM 100 mg L?1 pyrene. Finally, introduced for sole or supplementary carbon source presence salt concentrations. Furthermore, are bioremediation contaminated environments.